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Inflammatory Bowel Disease


Live probiotics protect intestinal epithelial cells from the effects of infection with enteroinvasive Escherichia coli (EIEC)
Resta-Lenert S, Barrett KE
Department of Medicine, School of Medicine, University of California-San Diego, UCSD Medical Center 8414, 200 West Arbor Drive, San Diego, CA 92103, USA. srestalenert@ucsd.edu


BACKGROUND:
The colonic epithelium maintains a life long reciprocally beneficial interaction with the colonic microbiota. Disruption is associated with mucosal injury.

AIMS:
We hypothesised that probiotics may limit epithelial damage induced by enteroinvasive pathogens, and promote restitution.

METHODS:
Human intestinal epithelial cell lines (HT29/cl.19A and Caco-2) were exposed to enteroinvasive Escherichia coli (EIEC 029:NM), and/or probiotics (Streptococcus thermophilus (ST), ATCC19258, and Lactobacillus acidophilus (LA), ATCC4356). Infected cells and controls were assessed for transepithelial resistance, chloride secretory responses, alterations in cytoskeletal and tight junctional proteins, and responses to epidermal growth factor (EGF) stimulation.

RESULTS:
Exposure of cell monolayers to live ST/LA, but not to heat inactivated ST/LA, significantly limited adhesion, invasion, and physiological dysfunction induced by EIEC. Antibiotic killed ST/LA reduced adhesion somewhat but were less effective in limiting the consequences of EIEC invasion of cell monolayers. Furthermore, live ST/LA alone increased transepithelial resistance, contrasting markedly with the fall in resistance evoked by EIEC infection, which could also be blocked by live ST/LA. The effect of ST/LA on resistance was accompanied by maintenance (actin, ZO-1) or enhancement (actinin, occludin) of cytoskeletal and tight junctional protein phosphorylation. ST/LA had no effect on chloride secretion by themselves but reversed the increase in basal secretion evoked by EIEC. EIEC also reduced the ability of EGF to activate its receptor, which was reversed by ST/LA.

CONCLUSIONS:
Live ST/LA interact with intestinal epithelial cells to protect them from the deleterious effect of EIEC via mechanisms that include, but are not limited to, interference with pathogen adhesion and invasion. Probiotics likely also enhance the barrier function of naive epithelial cells not exposed to any pathogen.




Comments from healthcare professionals who have used LB17:

Canada
"... I have been using different probiotic blends over the years. The best I found by far is LB17. The ... more strands of different friendly bateria, the better the probiotic. It has been my experience that LB17 covers every area of body fluids from the sinuses to the reproductive organs which give LB17 a great advantage over any other probiotic. My patients love the small size of the capsules and I have used LB17 with great success on open wounds and stubborn acne by ... applying ... directly to the wound.

... be happy to recommend LB17 to any health practitioner."..
Dr. A.W. Martin DC, Ph.D., RNCP, D.N.M.
(November 2007)
Dr Martin is a practising nutritionist in Sudbury Ontario with a PhD in nutrition.

United Kingdom
"I am a Holistic Therapy Practitioner in the UK .......

Finally, I want to make it clear that I am extremely pleased with the benefits that this product LB17 provides for myself and my clients and friends who all wish to purchase more of it..."

Ms Helen Rushforth B.A.(Hons), M.A., Flintshire, North Wales, UK
(May 2008)
Ms Helen Rushforth is a practising healthcare professional in North Wales, UK


Below is an image of a box of LB17 live probiotic and vegetable soft gel capsule
   
Each box contains 60 capsules


LB17 live probiotic biomass
LB17 is now also available in paste form without capsules for those who do not or cannot take capsules - see below

   
Above is a 50gm bottle of LB17 biomass. Next pic is image of the biomass in bulk paste form





The above information is provided for general educational purposes only. It is not intended to replace competent health care advice received from a knowledgeable healthcare professional. You are urged to seek healthcare advice for the treatment of any illness or disease.
The Food Standards Agency (UK) has not evaluated these statements. This product is not intended to diagnose, treat, cure, or prevent any disease.



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